AN ANALYTICAL FRAMEWORK FOR THE EXPLORATION OF ENDOGENOUS PEPTIDES INTEGRATING PEPTIDE STRUCTURE PREDICTION AND TANDEM MASS SPECTROMETRY

摘要

Peptidomics, an analytical workflow for systematic identification of short, native and endogenous peptides, has been widely used to study peptides in a wide variety of biological matrices. The workflow of peptidomics is largely diverted from proteomics, including LC-MS/MS and spectrum matching algorithm. However, sensitive and reliable identifications for endogenous peptides are still difficult even with recent mass spectrometry instruments.