WHAT DETERMINES THE POST-IONISATION EFFICIENCY IN MALDI-2: A COMBINED SOFT/HARDWARE-BASED SET-UP TO SEMI-AUTOMATICALLY CHARACTERIZE THE ROLE OF FIVE RELEVANT INPUT PARAMETERS

摘要

MALDI with laser-induced post-ionization (PI), also named MALDI-2, was recently introduced by us to increase ion yields for numerous classes of lipids, metabolites and carbohydrates [1]. Here we present a semi-automatic protocol to investigate and optimize the efficiency of the PI process in dependence of five relevant input parameters: pulse energies of the two lasers, PI laser wavelength, delay between the laser pulses, and buffer gas pressure in the ion source.