TANDEM MASS SPECTROMETRIC SEQUENCING OF ISOMERIC METHYLNUCLEOSIDES ON RNA TOWARD THE UNDERSTANDING OF EPITRANSCRIPTOME

摘要

The discovery of dynamic post-transcriptional modifications (PTMs) of ribonucleic acid (RNA) including N6-methyladenosines affects transcriptome, emerging field of 'epitranscriptomics'. Although its analysis requires a general sequencing method of modified nucleosides on RNA, conventional methods fail to provide such information. We present here an MS3-based method to sequence positional isomers of methylnucleosides in RNAs.