LILBID-MS REVEALS INFLUENCES OF THT AND COOMASSIE ON AGGREGATION KINETICS OF AMYLOID-BETA (1-42) AND α-SYNUCLEIN

摘要

Many proteins can spontaneously self-assemble into β-sheet-rich fibrillar aggregates, so called amyloids. Two examples are amyloid-beta (1-42) (Aβ42) and α-Synuclein (α-Syn), the proteins of interest regarding Parkinson's disease (PD) and Alzheimer's disease (AD), respectively. [1] To analyze aggregation of amyloids, a commonly used method is the Thioflavin T (ThT)-assay. Coomassie - used for staining proteins - is another important dye for protein analysis.