PROTEIN ANALYSIS USING ION MOBILITY MS AND IN-SOURCE ION ACTIVATION COMBINED WITH ECD ION FRAGMENTATION

摘要

Tandem mass spectrometry is accepted as a powerful analytical technique and when used with electron capture dissociation (ECD) makes it well suited for protein identification. In-source ion activation can be used to facilitate collision induced unfolding (CIU) of proteins and when coupled to IM-MS offers information about ion energetics and structures [1]. Combining the above technologies may provide topdown protein analysis with improved sequence coverage.