SHEDDING LIGHT ON STRUCTURE: INITIAL PROTEIN UNFOLDING EVENTS REVEALED BY 213 NM UVPD

摘要

Protein unfolding in its initial stages may be reflective of the stability of the entire fold and reveal local sites that may be perturbed. Ion mobility mass spectrometry (IM-MS) provides insight into global structure while UV photodissociation (UVPD) has been shown to be sensitive to secondary structural elements [1,2,3]. In this work we investigate the initial unfolding of three proteins using 213 nm UVPD combined with IM-MS.