A novel freestanding ultra-nanocrystalline diamond membrane for protein mass detection using MALDI-TOF-MS

摘要

Our data demonstrate the feasibility of the diamond nanomembrane detector-equipped MALDI-TOF-MS for protein analysis over a broad mass range. The results by the diamond-based detector outperformed those obtained from SiNx-and SOI-, and MCP-detectors. Ion detection by the nanomembrane-detector only depends on the kinetic energies of the impinging ions, providing ion-mass independent signal intensities. We expect further data demonstrating that this technology is a powerful tool for macro biomolecule (protein/complex) analysis such as virus capsids and protein aggregates.