Breeding of a homozygous two-type line with orange flower of dominant genic male sterility in Brassica napus L.

摘要

A cross between a sterile plant of the heterozygous two-type line Huyou1 5AB and the restorer line HF03 of dominant genic male sterility was made in Brassica napus L., All plants of F1 were fertile. In 6 lines ofF3 generation, fertility segregation was observed in five selfing populations. The progeny of 04-4307 had total 131 plants: 70 fertile plants with yellow flowers, 30 fertile plants with orange flowers, 22 sterile plants with yellow flowers, and 9 sterile plants with orange flowers. This F3 progeny segregated in a 3:1 ratio for fertile and sterile plants and in the same ratio for yellow and orange flower plants. The homozygous two-type line with orange flower is developed from sib-mating in this progeny among which the sterile and fertile plants have the genotypes MsMsrfrfy1y1y2y2 and MsMsRfrfy1y1y2y2, respectively.