Hybrid breeding of winter oilseed rape by utilization of self-incompatibility and cytoplasmic male sterility systems

摘要

Winter oilseed rape is the major oilseed crop in the Czech Republic. Several foreign hybrids based on male sterility systems MSL originated from Germany and Ogu-INRA from France is registered in our country at present.Three self-sterility systems including self-incompatibility (SI), cytoplasmic male sterility (CMS) Ogu-INRA and Shaan 2A are used for breeding of domestic rapeseed hybrids. The original self-incompatible lines developed from older European cultivars of rapeseed were characterized by a high glucosinolate (GSL) and erucic acid content. SI lines with improved seed quality have been developed by crossing of original SI materials with donors of double zero quality and subsequent deriving of doubled haploids (DH). Molecular markers based on PCR analyse of SLG gene have been developed for screening of self-incompatible genotypes. Comparison of seed test and molecular analysis of microspore regenerants for detection of SI and SC genotypes showed the possibility to discard majority of self-compatible plants in the early stage of development. Two different CMS systems are also used in breeding of hybrid cultivars. In Ogu-INRA CMS initial material obtained from France and Shaan 2A CMS from China, male sterile lines with improved seed quality were achieved by repeated backcrossing with DH donors of 00 character. The relevant fertility restorers (Rf) were also improved by means of crossing with 00 quality donors and subsequent development of doubled haploids.