Development of New Microfluidic Immunoassay Chips

摘要

This paper reports a new platform for chip-based immunoassay using photopolymer and antibody- immobilized small-sized polystyrene beads (1μΦ). Hydrogel pillars, which contain the antibody-immobilized polystyrene beads, in microchannels were fabricated by photolithography. By using this format, we realized (i) rapid analysis (a few minutes), (ii) easy handling (no need pump and valve), (iii) low-cost (disposable), (iv) small volume (1 droplets (0.25μL) of sample and reagents), and (v) high sensitivity.