Quantitative LC-MS/MS method development and preliminary analysis of plasma endocannabinoid concentrations in humans

摘要

In this study we established a sensitive LC-MS/MS method enabling efficient analysis of endogenous endocannabinoids in plasma, and evaluated two sample preparation procedures. Even though liquid-liquid extraction yield with toluene was slightly higher than with SALLE, SALLE was more reliable and reproducible across wide concentration ranges. Levels of AEA and 2AG detected in control plasma samples were within range of literature values. Isomerization of 2AG to 1AG was observed in all biological samples, regardless of extraction procedure used. The conversion rate was up to 60% in 40 hours. Levels of both 2AG and 1AG were measured.