The Use of Methionine Sulfoxide Reductases to Reverse Oxidized Methionine for Mass Spectrometry Applications

摘要

Methionine oxidation can be reversed in intact proteins and peptides prior to analysis by SDS-PAGE and intact or peptide LC-MS analysis. Reversal of methionine oxidation by MetSR has benefits to MS experiments involving intact proteins, targeted quantitative peptide analysis, and shotgun proteomics. A modified LC-MS ionization source design may alleviate the residual modifications observed at the protein and peptide level.