Lipid Peroxidation Profiles in Acute Hepatic Ischaemia-Reperfusion Injury

摘要

1. SWATH data independent acquisition techniques are suitable for the identification of lipid peroxidation products and metabolites of polyunsaturated fatty acids. 2. Lipid peroxidation profiles in acute hepatic ischaemia-reperfusion injury have been determined using data independent acquisition and targeted MRM techniques. 3. Liver ischaemia-reperfusion injury results in significant sustained in vivo elevation in isoprostane E_2, which persists in the ischaemic reperfused lobe for at least 28 days after clamping, while F_2 isoprostane levels remain unaltered. 4. E_2 and F_2 isoprostanes are formed competitively. Depletion of cellular reducing agents favours the formation of E_2 over F_2 isoprostanes.