Bioanalytical Quantitation Method for Analysis of 1,25-Dihydroxyvitamin D3 (DHVD3) in Human Plasma Using Amplifex Reagent Kit on SCIEX Triple Quad 5500 System

摘要

A highly selective, sensitive and high-throughput bioanalytical method was developed and validated for the detection of low levels of the DHVD3, in human plasma on the SCIEX Triple Quad 5500 LC/MS/MSSystem. Amplifex reagent showed exceptional ability to derivatized DHVD3 and increased the sensitivity to many folds and showed good reproducibility. Method sensitivity for DHVD3 detection was exceptional (2.59 pg/mLin plasma), and demonstrated high-reproducibilityand time effectiveness with good precision and accuracy. Analyte recovery is 102.12% for DHVD3 and 102.34% for DHVD3-d6 (IS).