Performance Evaluatio of the Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer for High-Throughput Top-Down Proteomics

摘要

Q Exactive HF mass spectrometer with its Intact Protein Mode and 1.8 fold increase in resolving power enables high-res analysis at high detection speed which makes it more suitable for high throughput top-down analysis. Aided by a new charge assignment/deconvolution algorithm, Q Exactive HF MS provides significant proteoform and protein coverage, even from a single direct infusion spectra. A multiplex SIM approach allows analysis of intact proteins up to about 50 kDa with isotopic resolution at LC timescale. 50 kDa with isotopic resolution at LC timescale.