Epitope Map of Malarial Antigen PvDBP revealed by Fast Photochemical Oxidation of Proteins (FPOP)

摘要

PvDBP (II): 2D10 Epitope Map FPOP and HDX determined epitope consistent with that of a subsequent X-ray determination of PvDBP:2D10 interaction using short chain variable fragment (scFv) construct Figure 5. Consensus of 2D10 epitope X. (A) Overall structure of the DBP-II/2D1O_(scfv) complex. DBP-II; scFv heavy chain (VH); light chain (VL) (B) Ribbon representation of DBP-II mapping the 2D10 epitopes. Improvements in FPOP epitope map fidelity can be achieved by normalizing total protein concentration (e.g., by using a nonbinding antibody).