In vivo Real-time Monitoring of Aphrodisiac Pheromone Release of Small White Cabbage Butterflies (Pieris rapae)

摘要

In this study, we have for the first time performed in vivo monitoring of male butterfly aphrodisiac pheromones released while flying in real-time. Our results indicate that the release of the aphrodisiac pheromones by male P. rapaemay be triggered by a visual cue. Using cDART-MS, it may be possible to clarify the role of specific triggers, and to localize the areas from which the pheromones are released on a wing. Our experiments clearly indicate that although some passive release may occur, a substantial increase in the release of the pheromones occurs when single males are mixed with other males or females of the same species. Further work is required to help better understand the release of aphrodisiac pheromones and determine the behavioral, chemical, or physical factors that are associated with their release.