Characterization of Kinetically Trapped Intermediates of Microperoxidase-11 Using TIMS-MS/MS and Molecular Modeling

机译：使用TIMS-MS / MS和分子模拟的微氧化酶-11中捕获中间体的动力学捕获中间体的表征

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The conformational dynamics of Microperoxidase-11 were explored as a function of solvent conditions and time after desolvation. Distinct IMS bands were observed. Their relative abundances were shown to depend on solvent conditions and time after desolvation. Kinetic profiles showed the interconvertion processes as a function of the desolvation time.

机译：作为溶剂条件和降解后的时间的函数探索微氧化酶-11的构象动态。观察到明显的IMS带。它们的相对丰度被证明取决于溶剂条件和降解后的时间。动力学曲线显示互连过程作为脱溶解时间的函数。

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2016年|1CD|共1页
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Jacob Porter; Alyssa Garabedian; Paolo Benigni; Jaroslava Miksovska; Francisco Fernandez-Lima;
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1. Shifting the Competition between the Intramolecular Reshuffling Reaction and the Direct Oxidation Reaction during the Oxidative Folding of Kinetically Trapped Disulfide-Insecure Intermediates. [J] . Narayan M, Welker E, Wanjalla C, Biochemistry . 2003,第36期

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2. Evidence for kinetic intermediate states during the refolding of GdnHCl-denatured MM-creatine kinase. Characterization of a trapped monomeric species [J] . Leydier C., Couthon F., Marcillat O., Biochemistry . 1998,第50期

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4. Characterization of Kinetically Trapped Intermediates of Microperoxidase-11 Using TIMS-MS/MS and Molecular Modeling [C] . Jacob Porter, Alyssa Garabedian, Paolo Benigni, ASMS Conference on Mass Spectrometry and Allied Topics . 2016

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5. Fourier transform ion cyclotron resonance mass spectrometry instrumentation and methods for structural characterization of trapped biomolecular ions: Innovative MS/MS techniques, gas-phase hydrogen /deuterium exchange, and laser -induced fluorescence. [D] . McFarland, Melinda A. 2004

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Characterization of Kinetically Trapped Intermediates of Microperoxidase-11 Using TIMS-MS/MS and Molecular Modeling

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