Democratizing and expanding the reach of DIA Mass Spectrometry: Developing OpenSWATH tools and workflows within user-friendly Galaxy-P platform

摘要

Data independent acquisition (DIA) combines the advantages of shotgun proteomics and selected reaction monitoring approaches; which generate continuous, highly complex ion fragmentation data. Researchers can analyze the data immediately, or archive it for a hypothesis-driven re-analysis later. OpenSWATH software addresses challenges of DIA data analysis, offering transparency, vendor neutrality and usable output.