Online Hydrophobic Interaction Chromatography-Mass Spectrometry for Top-Down Proteomics

摘要

The new HIC combination works as an intermediate between HIC and RPC. It requires some organic solvent and denatures certain proteins to some degree. The function of the salt seems to be to preserve protein structure rather than promote retention. The less hydrophobic materials PolyPENTYL A, PolyHEXYL A and PolyHEPTYL A offer the promise of elution of proteins in high yield and reasonably well-shaped peaks with retention of at least some tertiary structure. We have successfully demonstrated the feasibility of online HIC-MS for top-down proteomics.