Evaluation and Comparison of Nonderivatization and Derivatization Tandem Mass Spectrometry Methods for Multianalyte Analysis in Dried Blood Spots

摘要

Flow injection-tandem mass spectrometry methods were developed to simultaneously detect and quantify amino acids, acylcarnitines, and succinylacetone in a single extraction process in dried blood spots for research. Rapid data processing was performed using iRC PRO metacalculation software. Both nonderivatization and derivatization sample preparation methods were capable of accurately quantifying AA/AC/SUAC on a TSQ Endura triple quadrupole MS with a run time of 1.5 min. The TSQ Endura MS can provide within-run imprecision (n=10) at three enriched concentrations of less than 10% and run-to-run precision (n=70) of less than 15% for both nonderivatization and derivatization methods. The method difference between quantitative values resulting from nonderivatization and derivatization methods was minor and both methods are highly correlated.