Towards Global Sulfation Analysis: Integrating Weak Anion Exchange and Ultraviolet Photodissociation Mass Spectrometry with Strategic Modulation of Peptide Basicity

摘要

193 nm UPVD enables sequence characterization and PTM localization in sulfopeptide anions Sulfopeptide affinity for WAX is greatest when (1) #sulfates is increased (2) #basic sites is decreased and (3) #acidic sites is increased, with trends ranked in order of impact Carbamylation blocks ionizable sites on lysines and N-termini to remove interfering positive charges and consequently improve sulfopeptide retention on WAX Sulfopeptide separation from a matrix of BSA peptides was achieved using the carbamylation WAX strategy