Analysis of Angiotensinogen Redox Switch Involved in the Pathogenesis of Pre-eclampsia by Targeted LC-MS/MS

摘要

A reliable level of enrichment for AGT from plasma was achieved, applying a new economic two-step fractionation approach with high throughput potential by a combination of lectin affinity chromatography and RP-SPE. The two forms of AGT (oxidised and reduced) involved in the redox switch were detected from plasma chymotryptic digest. by differential alkylation approach coupled to targeted LC-MS/MS.