Proteomic and Transcriptomic Analysis of a Solvent Producing Bacterium Clostridium acetobutylicum ATCC 824

摘要

Clostridium acetobutylicum (Cac) is a Gram-positive, endospore-forming anaerobic bacterium and it has received lot of attention for its production of industrial chemicals and fuels, including butanol, ethanol, butyrate, isopropanol, acetoin and acetone. These metabolites can impose toxic effects on cells and might impact cell growth and metabolism. The mechanisms through which Cac adapts to metabolite stress at the systems level are not well known. The complete of Cac genome sequence and the development of genomic and proteomics technologies make the omics techniques ideal tools to study global changes after metabolite stress. In this study, iTRAQ and microarray techniques were employed to understand the transcriptomic and proteomic changes upon butanol and butyrate stress.