Targeted Protein Expression Profiling using MRM: Genetic vs. Environmental Variation of Plasma Protein Levels using a Twin Cohort

摘要

Solutions to three significant biomarker verification challenges have been demonstrated. (1) MRM scheduling extends multiplex capability of MRM assays (>500) without compromising analyte utilization. (2) Rapid MRM assay development strategies using the MIDASTM workflow on the 4000 QTRAP~R system. (3) Quantitative accuracy is enhanced with use of global reference sample and mTRAQ~(TM) reagents. Analytical reproducibility is very high, percentCV of <5percent for many peptides in human plasma. Cost effective internal standard for large, multi-instrument, multi-lab studies. Twin study data processing still in process, lots of information to be mined from dataset. (1) Range of biological variation observed for different plasma proteins (2) Twins typically possessed much higher similarity in plasma protein profile to each other than to non-related individuals. (3) Accuracy of MRM approach seems high enough to be able to measure small protein flucuations.