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Mass Spectrometric Analysis of Thiocitrulline Bound Proteins Extracted from Human Keratinocyte Cells

机译:从人角蛋白细胞中提取的硫酸酯结合蛋白质的质谱分析

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IDA files were searched against Swiss-Prot database using Protein Pilot and Mascot search engines. Overall, 9 proteins were detected. Two of them were contaminant proteins (serum albumin and keratin). Six of the nine proteins were found by both search engines. Two proteins were only found from the Paragon search and one unique protein was found only from the Mascot search. A control experiment was also performed to identify any non-specific binding. From the control experiment, one protein was found from the both search engines. The Paragon search engine yielded one more protein.
机译:使用蛋白飞行员和吉祥物搜索引擎搜索瑞士-PLATA数据库的IDA文件。总体而言,检测到9个蛋白质。其中两个是污染蛋白质(血清白蛋白和角蛋白)。搜索引擎发现了六种九个蛋白质。只有两种蛋白质从帕拉格搜索中发现,只能从吉祥物搜索中找到一个独特的蛋白质。还进行对照实验以鉴定任何非特异性结合。从对照实验中,从两个搜索引擎中发现了一种蛋白质。 Paragon搜索引擎产生了一种蛋白质。

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