Electron Capture Dissociation of Metal-Adducted N-linked Oligosaccharides Released from Bovine Thyroid Stimulating Hormone

摘要

First application of ECD to the characterization of N-linked glycans released from a glycoprotein is demonstrated. IRMPD and ECD generate complementary product ions, including both glycosidic and cross-ring cleavages. Labile sulfate groups in sulfated N-glycans were retained in both ECD and AI-ECD. However, AI-ECD showed more extensive fragmentation. ECD of different metal adducted species showed different fragmentation patterns. Calcium adducts yielded higher fragmentation efficiency in both IRMPD and ECD compared to the other metal adducts.