With minimal, targeted changes in primary amino acid sequence, peptides from the coiled coil domain of fibrin can be designed to fold into multimeric coiled coil bundles. Moreover, the peptides were not found to be cytotoxic in cell culture. In our next experiments, we will utilize peptide γ_(52-88)KI as a basis for forming well-folded self-assembled biomaterials by producing multi-arm peptides and peptide polymers capable of forming networks. These peptides will serve as a basis for constructing three-dimensional self-assembled scaffolds and for tailoring the specific bioactivity of these scaffolds to achieve desirable cell and tissue responses.
展开▼
