Effect of Human Fibroblasts Over the Osteoblastic Activity of Human Osteoblastic-like Cells: Role for Cell-Cell Interactions

摘要

The developed co-culture model showed that SaOs-2 proliferation was not directly or indirectly affected by MRC-5 cells. In addition, MRC-5 inhibited SaOs-2 in vitro ALP production through direct cell-cell contact and in particular through gap junctional communication. The cellular interactions proven to occur, render this in vitro co-culture model as a useful tool for a better understanding and eventually controlling of the cellular response to in vitro cultured tissue engineering scaffolds.