Effect of ischemic postconditioning on the oxidative stress and tissue injury in the intestinal warm ischemia/reperfusion model

摘要

Intestinal ischemia/reperfusion (I/R) injury is resulting from some pathological and surgical procedures. Ischemic postconditioning (IPO) phenomenon was described as brief periods of reperfusion alternating with re-occlusion applied during the early minutes of reperfusion. We aimed to investigate the effect of IPO in the warm intestinal I/R model. Warm ischemia was performed by occlusion of superior mesenteric artery for 1, 3 and 6 hours on white domestic pigs (n=30). Reperfusion lasted 3 hours in all groups. Before reperfusion intestine were postconditioned by 3 cycles of 30-seconds ischemia and 30-seconds reperfusion. Small intestine tissue was collected after laparotomy (control) and at the end of the reperfusion periods. From oxidative stress markers tissue value of malondialdehyde (MDA) and reduced glutathione (GSH), and the activity of superoxide dismutase (SOD) were determined. Tissue damage was evaluated by quantitative method. IPO significantly decreased this injury in each group. IPO prior to reperfusion mitigated the oxidative stress and histological damages in our experimental warm I/R model.