The COP9 signalosome has a role in adipogenesis

摘要

Obesity is one of the most serious health problems of the 21st century. The expansion of fat tissue requires angiogenesis, which needs the vascular endothelial growth factor (VEGF), a protein controlled by the COP9 signalosome (CSN). Methods: LiSa-2 cell line was used as a model for adipogenesis. Insulin (INS), cortisol (COR) and triiodthyronine (T3) stimulated the differentiation of LiSa-2 adipogenesis. Ten uM of curcumin (CUR) were used to inhibit the CSN and to block VEGF production. LiSa-2 cells were transfected with siCSN5 and CSN5wt or CSN5 mutant. VEGF production was quantified by ELISA. Changes of regulatory proteins were assessed by Western blotting. Results: CUR affected protein levels of p53 and c-Jun in LiSa-2 cells moderately. However, the VEGF production was significantly reduced. Overex-pression of CSN5 increased the VEGF production by 30%, whereas downregulation of CSN5 led to a reduction of VEGF. Conclusion: The CSN is a potential regulator of adipogenesis.