Study on Effects of Pulsed Magnetic Fields on Proliferation and Differentiation of an Osteoblast-Like Cell Line (SaOS-2)

摘要

To study of the bioeffects of electromagnetic fields (EMF) is an important task in biological physics. Using EMF to treat bone disease involves electrical technology, biology, and medicine, but the effects of EMF are still controversial and the mechanisms are yet widely unexplained. Therefore, more effort is needed to discover the effects of EMF at cellular and molecular levels. In this paper, the effects of low-frequency pulsed magnetic fields (PMF) on proliferation and differentiation of osteoblast-like cell line (SaOS-2) were investigated. The results show that PMF with frequency 15 Hz, magnetic induction density 0.4～0.7 mT and exposure time 3 or 6 hours per day has the best enhancement effect on proliferation of cells. Impressively, PMF also played important role in differentiation of osteoblast. The effect on promoting differentiation level was proved by measuring ALP activity.