DEMONSTRATION OF TRI-COLORED REPORTER ASSAY USING A FILTER LUMINOMETER 'SIRIUS C'

摘要

A reporter assay system to monitor the expression of several genes simultaneously is required to investigate a cascade of transcriptional regulations, which is the molecular basis of various biological functions. Recently, we have developed a novel reporter assay system, a tricolor reporter in vitro assay system, in which the expression of three genes can be monitored simultaneously by splitting the emissions from green-, orange-, and red-emitting luciferases with an optical filter.1 In this study, we demonstrated this novel system to analyze a transcription-translation feedback loop of c-fos promoter using a luminometer "Sirius C" fitted with optical filters.