Characterization of Low Molecular Water Soluble Chitosan Nanoparticles to Carry of Anticancer Agent

摘要

Low molecular and water soluble chitosan nanoparticles (LMWSC-NPs) were prepared by dialysis method as modify the chitosan with hydrophobic and hydrophilic group and investigated the potential of application as delivery system. The LMWSC-NPs modified hydrophobically would be used to both of intravenous injection and oral administration of hydrophobic drugs due to their adequate size for administration. We measured NMR, IR, and photon correlation spectroscopy (PCS) to investigate the characteristics of the nanoparticles. The synthesis of hydrophobically modified-chitosan nanoparticles was identified from the results of 1H-NMR, IR spectrum. From the results of surface morphology observed by TEM and AFM, spherical nanoparticles were identified and its size was 30～ 100nm. At PCS measurement, the more increase the number of substitutive group, the more decrease the positive charge of nanoparticle surface.