Genetic diversity of hull-less barley (Hordeum vulgare L.) Iandraces in the highlands of central Nepal as revealed by SSRs

摘要

A set of 107 naked barley genotypes from mountains and highlands of Annapuma, Manaslu and Lamtang Himalaya range in central Nepal and 8 selected German and Canadian cultivars were analysed for genetic similarity using 40 SSRs covering the barley genome. Based on this, 258 alleles were detected with an average of 6.45 alleles per SSR. Taking into account only Nepalese barley on average 4.8 alleles were found. The overall diversity index was estimated at 0.53 and within Nepalese Iandraces at 0.52. TheUPGMA clustering based on Dice similarity coefficient clearly separates Nepalese Iandraces from European and Canadian cultivars. The Nepalese collection is grouped into two distinct clusters and several sub-clusters, which in most cases are in accordancewith the collection sites. The SSR markers revealed considerable genetic diversity within the Nepalese naked barley Iandraces.