Analysis of Cerebrospinal Fluid for the Detection of Ruptured Cerebral Aneurysm using Spectrophotometry and Signal Processing Techniques

摘要

An accurate quantification of bilirubin in cerebrospinal fluid (CSF) will provide a simple, sensitive and rapid mechanism for detecting subarachnoid hemorrhage (SAH) and for its differentiation from a traumatic spinal tap. Derivative analysis of the spectrophotometric data provides a model for determining bilirubin in CSF where the primary contaminant is Methemoglobin. Bilirubin values are determined in the range 0-9mg/dl within a methemoglobin concentration of 4.6g/dl using the derivative analysis method. The algorithm is also implemented on test samples in which the bilirubin value is constant (4.6mg/dl) and the methemoglobin varies between 0-9g/dl. The performance of the derivative analysis method is compared to the modified minimum distance method developed in. We suggest a combination of these methods for accurate bilirubin estimation in CSF/hemoglobin. This will provide the foundation for the development of a portable user friendly device for diagnosis of SAH.