Characterization of Mn-peroxidase Isoenzymes from the White-rot Fungus Trametes versicolor in Korea

摘要

The white-rot basidiomycete Trametes versicolor produced high levels of Mn-peroxidase activity when grown on Kirks media with 0. 05% Tween 80, 2. 4 mmol/L (NH_4)_2-tatrate, 80 mg/kg of MnSO_4. Maximum activity of Mn-peroxidase was appeared on the 10th day of culture on above medium of combinations. Two types of isoenzymes of Mn-peroxidase were separated by DEAE-Sepharose CL-6B, SP-Sepharose, anion and cation exchange chromatography on Mono-Q and Mono-S column. Those iso-enzymes were characterized in view point of enzyme kinetics, N-terminal amino acid sequences, and base sequences of the coded genes, etc.