A High Performance Monolithic Column for Bio-chromatography

摘要

A novel approach for the fabrication of macroporous poly(glycidyl methacrylate-ethylene glycol dimethacrylate) (GMA-EDMA) monolith is presented. The method involves the use of Na2SO4 granules and organic solvents as co-porogens. Compared with the conventional monolith (ML) using organic solvent only as porogen, the improved monolith (MLS) not only have higher dynamic binding capacity (DBC) for protein (bovine serum albumin, BSA), but also have lower backpressure and higher column efficiency. Moreover, poly(glycidyl methacrylate-diethylamine) tentacles were grafted onto the pore surface of MLS monolith. This has further increased the DBC of BSA to 74.7 mg/ml, about two times higher than that of the monolith without the grafted tentacles. Also, this grafting did not obviously decrease the column permeability. Hence, a new monolith of high column permeability and binding capacity was produced for high-performance preparative protein chromatography.