Type II collagen peptides for measuring cartilage degradation

摘要

This paper describes two new immunoassays for a peptide of the triple helix of type II collagen (Coll 2-1) and its nitrated form (Coll 2-1 NO_2) In healthy subjects aged between 20 and 65 years old, Coll 2-1 and Coll 2-1 NO_2 levels in serum were in means 125,13 +- 3.71 and 0 16 +- 0,08 nmol/1, respectively. These levels did not significantly vary with age. However, up to 45 years of age, Coll 2-1 NO_2 levels in women were significantly higher than in men,. In patients with knee osteoarthritis (OA), Coll 2-1 in serum was found to be elevated compared to healthy controls (267,45+-26,,42 nmol/1 vs 126,78+-6,,61 nmol/1). Further, we have demonstrated that an increase of the urinary levels of Coll 2-1 or Coll 2-1 NO_2 over 1 year was predictive of joint space narrowing progression in OA patients. In conclusion, these preliminary results indicate that Coll 2-1 could be a predictive marker of knee OA progression.