Application of Siderophore Receptor Proteins and Porins Technology for Controlling Klebsiella Mastitis in a Commercial Dairy Herd

摘要

Nearly all bacteria require iron to grow, and iron acquisition systems are known to be conserved across many pathogens. To facilitate iron uptake, bacteria release highly specific transport systems called siderophores that have high affinity for ferric iron and can strip it away from host binding proteins. For each type of siderophore, bacteria express a complimentary outer membrane siderophore receptor protein (SRP~R) that facilitates transport of the iron-siderophore complex through the bacterial outer membrane. For the production of a Klebsiella SRP vaccine, Klebsiella is grown under iron restriction in large scale fermentation mimicking the low iron encountered during natural infection of the host. This causes the bacteria to express iron acquisition systems to scavenge for iron during fermentation. The bacterial culture is then harvested and processed through an extensive purification process, yielding a highly purified and concentrated preparation of iron regulated outer membrane siderophorereceptors and porin proteins.