Characterization of One-Pass and Pull-Length Sequences of Oligo-Capping cDNA Clones by Genome Mapping

摘要

Whole human genome sequence determination was completed this year. To obtain accurate gene structures and promoter information, full-length cDNA sequence information as well as the genome sequences is indispensable. Moreover, the full-length cDNA clones and sequences are valuable for functional analysis of genes. The oligo-capping cDNA library developed by Maruyama and Sugano is an effective source of the full-length cDNA clones. The full-length human cDNA sequencing project supported by New Energy and Industrial Technology Developmental Organization (NEDO) determined 30,000 full-length and more than one million 5' one pass cDNA sequences of oligo-capping clones obtained at HRI and Tokyo University. Consequently, the NEDO human splicing variant cDNAproject started in 2002, taking advantage of these oligo-capping cDNAs which are regarded as a valuable source of splicing variant cDNAs.