Fluorescence-enhanced tomographic imaging in large phantoms using gain-modulated ICCD camera

摘要

A frequency-domain photon migration (FDPM) imager employing an image-intensified CCD camera for fast data acquisition on a large tissue-mimicking phantom (1087 ml) is described. Fluorescence-enhanced imaging is performed employing frequency-domain techniques at 100 MHz in order to obtain the boundary measurements of phase and amplitude and to recover the interior optical maps using the first principles of light propagation. The effect of refractive-index parameter in the boundary condition of the light propagation model is not significant due to the large phantom volume and its curvilinear nature. Initial experiments were performed under perfect (1:0 contrast) and imperfect (100:1 contrast) uptake cases using indocyanine green as the contrast agent. Preliminary 3D image reconstructions using the approximate extended Kalman filter (AEKF) algorithm are presented.