Ultra-sensitive, diode laser-based monitor for singlet oxygen

摘要

In this paper we present results from experiments to develop a real-time, optical monitor for singlet molecular oxygen produced during photodynamic therapy. Using a pulsed diode laser and a sensitive photomultiplier tube, we have obtained signals from singlet oxygen during and following pulsed laser excitation. Several photosensitizers were used, and we obtained strong signals even in the presence of protein laden environments. Values obtained for the lifetimes of the singlet oxygen state and the photosensitizer triplet state are compared to literature values.