A transeriptome map of wheat

摘要

A transeriptome map of wheat genome, with 1;099 EST loci spanning the 21 chromosomes of bread wheat (Triticum aestivum L.) or an average of 766 loci per chromosome, has been developed. Of the 1;099 EST loci, 1;051 were allocated to 159 bins using a set of nullisomic-tetrasomic, ditelosomic, and deletion lines providing an expected average of 95 loci per chromosome bin or 1 EST locus per 1 Mb of wheat DNA. Observed EST distribution was non-random with 40% of the EST loci mapping in the bins located inthe distal 20% of the chromosomes. Most agronomic genes were located in the EST-dense distal bins. The wheat transeriptome map, together with the draft sequence of the rice genome, now provides unique opportunities for gene discovery in wheat. More importantly, this wheat bin map will be a resource for markers for breeding and framework for constructing a sequence-ready BAC-contig map of the wheat genome.