A REGULATION OF SPHINGOMYELIN LIPID CYCLE BY THE LIPOSOMES TREATMENT AT THE RATS HAVING EXPERIMENTAL HEPATITIS

摘要

Sphingolipids comprise indispensable structural components of all known eukaryotic plasma membranes they regulate the signal transduction elements including protein kinase C. Sphingosine, ceramide and other sphingolipid derivatives are also known to play central role in apoptosis, cell cycle regulation, inflammation and intracellular calcium mobilization. To get experimental inflammation of a rat liver the CCl{sub}4 was administered. During the process of experimental hepatitis development the Siposomes loaded by the modulators of a sphingomyeline Lipid cycle (SLC) - the sphingomyelin and ceramide were administed. There were two groups of experimental animals: I -toxic hepatitis (control) and II - group of toxic hepatitis rats with the liposomes administed. The inflammation stage of a liver was determined as activity of alaninaminotransferase (ALT), bilirubin level and thymol test. The components of a SLC change were tested by the contents of a sphingomyelin, ceramids and activity sphingomyelinase. The increase of activity sphingomyelinase and Increase of the contents of ceramides at the rat's liver was revealed, The liposomes administed into rat trail vien leaded to the ALT activity decreases and the bilirubin contents reduced. Thus a result indicates the decrease of an inflammation process of a liver tissue. The morphological research of a liver tissue confirmed the reduction of hepatitis activity during the liposomes entered. The injects of liposomes loaded the SLC's modulators reduced the activity the inflammation process of liver tissue.