NOVEL SURFACE MODIFICATION METHODS AND SURFACE PROPERTY ANALYSIS FOR SEPARATION OF DNA BIO-MOLECULES USING CAPILLARY ELECTROPHORESIS

摘要

This paper presents systematic investigation on the surface properties of electroosmotic flows (EOF) inside microchannels for quartz, glass and PDMS based materials. Two novel methods to modify the surface properties of glass-based microchannels for capillary electrophoresis (CE) are developed. Instead of using complicated and time-consuming chemical silanization procedures for surface modification of the CE channels, two simple and reliable methods utilizing organic-based spin-on-glass (SOG) and water-soluble acrylic resin are reported, providing a fast and batch process for surface modification of glass-based CE channels. The proposed methods are evaluated using separation of ΦX-174 DNA makers. Experimental data show that separation efficiency is greatly improved. In addition, long-term stability of the SOG coating is also verified in this study.