SINGLE MOLECULE AMPLIFICATION IN A CONTINUOUS FLOW LABCHIP DEVICE

摘要

We have developed an integrated system to perform automated, high-throughput, continuous flow PCR in a microfluidic sipper chip. The system is unique in several respects. One, the system integrates reaction assembly, thermal cycling and fluorescence detection on one chip. This allows different samples to be tested one after another in an automated way, all at nanoliter scale. Two, the chip we have created uses our sip-and-split design and has eight channels in which eight different loci can be amplified at one time for each sample. In addition, we have recently configured the system to continuously amplify and detect single molecules of DNA.