The impacts of CO_2 ocean sequestration on microbial communities and their metabolism are of great concern because they play an important role in the biogeochemical cycles. A mesocosm experiment was conducted to study the responses of marine microbial communities to different concentrations of CO_2 . Seawater was delivered to four tanks on land from the offshore, using a tube pump. Four CO_2 conditions of experimental ecosystems were formed by CO_2 enriched air under dark conditions. Chemical and biological parameters were analysed to evaluate the impacts of CO_2 on the microbial communities. The biomass of each tank was not affected considerably by the different CO_2 conditions, but the potential activities of ectoenzyme were decreased under high concentration of CO_2 . The effects of CO_2 on bacterial communities were also confirmed using molecular biological methods. The present studies were carried out in short term and limited conditions using biota of estuarine. Further experiments are needed to generalize the impacts of CO_2 on the marine ecosystem.
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