SYSTEM IDENTIFICATION TO ANALYSE CHANGED KINETICS OF SERCA IN INTACT RAT HEART

摘要

A mechanistic-based model has been derived of calcium handling in the intact heart. This model incorporates the quantitatively most important processes involved in beat-to-beat calcium homeostasis. Based on a priori physiological information the model has been reduced to yield (kinetic) parameters that could be estimated using time-series data of the free calcium concentration in the sarcoplasma. Observations of the dynamics of the overall system were translated into the underlying mechanisms. Experiments in which the most important calcium extrusion pump (Sarcoplasmic Reticulum Ca~(2+)-ATPase, SERCA) was disturbed have been successfully analysed and interpreted using model and identification.