COMPARTMENTAL MODELLING TO ASSESS STABILITY OF TOPOTECAN BOUND TO DNA

摘要

In this paper, a compartmental model is proposed for describing the in vitro kinetics of the anti-cancer agent topotecan following administration into a culture medium containing human lymphoma cells in suspension. The model extends one previously proposed by including an extra dissociation pathway, with the objective of describing the effect of DNA binding on the stability of bound topotecan. The structural identifiability and steady states of the model are analysed. The model is fitted to high performance liquid chromatography data and it is found that there is a qualitative difference between the predicted behaviour of the new model and that of the previous model.